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First, single molecule events obtained from all frames are plotted together (red dots) and over-imposed on the membrane stain image (white).

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Pre-publication history The pre-publication history for this paper can be accessed here: Acknowledgements This study is funded by a grant from the funding body ZonMw, the Netherlands buy slim tea fedex Organization for Health Research and Development (project number: 80-82435-98-8058). After the telophase, these fragments may not be included in the nuclei of daughter cells, and form single or multiple micronuclei in the cytoplasm (OECD 1997 ). This in vivo imaging technique was applied to the zebrafish together with the gene transfer method to inject plasmid DNA into the early embryo.

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