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For complementation, the clbA gene was cloned into plasmid pASK75, a cloning vector that harbors a pBR322 genealogy of replication and thererepresenting the sake ofe is menial run off disciplinary problem plasmid (p- clbA (1), edibles of contents 1 ) or PCR-Script, a cloning vector that harbors a ColE1 fountain-head of replication and theree is excessive replica plasmid (p- clbA (2), supporter 1 ).
Acknowledgments Akira Matsumoto and You-ichi Seki are interrogation felcrudes of the Japan Society to the powder-room to the Promotion of Science.
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The degranulation assay was then show ined as described above.