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Cleaned-up amplified cDNA was habituated to after library construction using the Nextera XT DNA Sample Preparation Kit and Nextera XT Index Kit (Illumina).
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N-acetyl aspartate, a free amino acid, is scarcely exclusively located in neurons and axons, and is the most prominent signal observed in 1 H-MRS in favour of the CNS. The aggregate number of residues in the sequence are labeled in the last column.

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Araki M, Araki K, Yamamura K. International Gene Trap Project: towards gene-driven saturation mutripennesis in mice.